| Chicago Symphony Orchestra | Research and Development Fund | 12/06/12 | $2,500,000 | Chicago, IL, United States | Arts and Cultural Heritage |
| Institute of International Education, Inc. | Artist Protection Fund | 03/06/19 | $2,500,000 | New York, NY, United States | Arts and Cultural Heritage |
| Virginia Polytechnic Institute and State University | Symphony Orchestra Program | 06/21/02 | $2,500,000 | Blacksburg, VA, United States | Arts and Cultural Heritage |
| The School of American Ballet, Inc. | Diversity Initiative Endowment | 12/10/15 | $2,500,000 | New York, NY, United States | Arts and Cultural Heritage |
| LYRASIS | Emergency Preparedness Pilot | 12/08/16 | $2,500,000 | Atlanta, GA, United States | Arts and Cultural Heritage |
| Philadelphia Museum of Art | Scholarly Publications - Endowment | 03/15/02 | $2,500,000 | Philadelphia, PA, United States | Arts and Cultural Heritage |
| National Gallery of Art | Center for Advanced Study in the Visual Arts - Paul Mellon Fund | 03/27/86 | $2,500,000 | Washington, DC, United States | Arts and Cultural Heritage |
| Yale University | Art Conservation - Research Center | 12/01/11 | $2,500,000 | New Haven, CT, United States | Arts and Cultural Heritage |
| Musical Arts Association | Artistic Initiatives | 12/06/12 | $2,500,000 | Cleveland, OH, United States | Arts and Cultural Heritage |
| Settlement Music School of Philadelphia | Philadelphia Music Alliance for Youth Artists' Initiative | 12/09/19 | $2,500,000 | Philadelphia, PA, United States | Arts and Cultural Heritage |
| New York City Opera, Inc. | 20th and 21st Century Opera Projects and Productions | 06/13/08 | $2,500,000 | New York, NY, United States | Arts and Cultural Heritage |
| Victoria and Albert Museum | Victoria and Albert Museum - V&A Research Institute | 12/10/15 | $2,500,000 | London, United Kingdom | Arts and Cultural Heritage |
| National Arts Strategies, Inc. | General Support | 12/15/88 | $2,500,000 | Alexandria, VA, United States | Arts and Cultural Heritage |
| Philharmonic-Symphony Society of New York, Inc. | Artistic Initiatives | 12/06/12 | $2,500,000 | New York, NY, United States | Arts and Cultural Heritage |
| New Music USA, Inc. | Core Institutional Support | 12/15/00 | $2,500,000 | New York, NY, United States | Arts and Cultural Heritage |
| Settlement Music School of Philadelphia | Philadelphia Music School Alliance for Youth Artists' Initiative | 12/08/16 | $2,532,000 | Philadelphia, PA, United States | Arts and Cultural Heritage |
| National Performance Network, Inc. | Financial Health Initiative | 12/08/16 | $2,620,000 | New Orleans, LA, United States | Arts and Cultural Heritage |
| Philadelphia Museum of Art | Conservation Endowment | 09/15/00 | $2,625,000 | Philadelphia, PA, United States | Arts and Cultural Heritage |
| Carnegie Mellon University | Carnegie Mellon Research Institute | 03/16/98 | $2,630,925 | Pittsburgh, PA, United States | Arts and Cultural Heritage |
| The International Association of Blacks in Dance, Inc. | Comprehensive Organizational Health Initiative | 03/15/18 | $2,636,000 | Silver Spring, MD, United States | Arts and Cultural Heritage |
| Nonprofit Finance Fund | Financial Health Initiative | 12/10/15 | $2,650,000 | New York, NY, United States | Arts and Cultural Heritage |
| New York Shakespeare Festival | Public LAB and Shakespeare Outreach | 12/11/07 | $2,700,000 | New York, NY, United States | Arts and Cultural Heritage |
| The Art Institute of Chicago | Conservation Science Endowment | 12/15/00 | $2,750,000 | Chicago, IL, United States | Arts and Cultural Heritage |
| Institute of International Education, Inc. | Artist Protection Fund | 03/12/15 | $2,785,000 | New York, NY, United States | Arts and Cultural Heritage |
| George Eastman House | Conservation Endowment | 12/11/07 | $3,000,000 | Rochester, NY, United States | Arts and Cultural Heritage |
